The effect of pore structure of collagen scaffold on cultured human chondrocytes in cartilage tissue engineering

Abstract

Statement of the Problem: One of the most important problems in humans ,s damage to the cartilage of the Joints Of the common case  which affects  most people of different  ages  ,s osteoarthritis  that  is due to the  loss of the  articular  cartilage of the knee that in most cases, the subchondral bones in the cartilage subcutaneous are also damaged These lesions are not thoroughly curable due to the limited repair power ,n the normal cartilage  because of absence of lymph, vascular  and nerve tissue One of the newest remedies for cartilage treatment ,s making autologous cartilage ,n vitro and 1,nk,ng ,t to the patients Joint  By using tissue engineering methods, one can design a b1ocompat1ble scaffold, using natural prot"in and seed it to the patient's autologous chondrocyte cells and produce l1v1ng human tissue The purpose of this study is comparison  of  different  methods  in the freeze-drying  process of designing  and constructing  an appropriate  bio-scaffold with effecuve porosrty size and shape, so that the highest efficiency required for the implantation and proliferat1cn in the scaffold is created   Method  During a study, identical collagen  hydro gels and different  scaffolds  were  prepared by   different

conditions of freeze drying Then, the patien\'s cartilage was biopsied ,n accordance with ethical principles and chondrocyte cells were e,1racted and multiplied from the tissue,The cells were then placed adJacent to the scaffolds and cartilage tissues of different qualities were prepared 1n a month Findings  After   erform1ng spec1alize1j tests related to cartilage tissue, the scaffold which had a freeze dr,;,ng that reached -40 'C over a 90 minutes ambient temperature manifested the best car tilage tissue !01mat1on compared to the other groups Conclusion Human cells ,n the form of tt1ree--dimens1onal tissues in the scaffolds, be prepared with physical and chemical cond1t1ons in vitro and lead to fabrication of livir,g human cartilage tissues  and  De used in treatment  of diseases  associated  with cartilage damage

Biography

Mahdi Had, is currently working as Biological Medicines Director at Tof,gr Daru Research & Engineering Company in Tehran and ,s engaged  ,n developing  advanced  b,o-based pharmaceuticals  complying with GMP requirements   He has bee  involved 1n stem cell and tissue engineering 1n research and industrial cen1ers of  Iran for  12 years  He has worl<ed as  a

Researct1er  at Royan Institute, Tehran   He has worked as the Pl'OJect  Manager  of Cell Therap·,Med1c1nes  Produc1on and tissue engineering .

Health Desk An I ra nia n researcher was able to prod uce h uma n carti lage by sampling, proliferating and culturing huma n cartilage cells on a collagen protei n scaffold .

The plan's executor, Mahdi Had !, told ISNA that the production of human cartilage is one of his research achievements, adding the initial product was manufactu red  in  the laboratory and  it  can be produced industrially as a medici nal product in the near fut ure

Elaborating  on  his  project,  Hadi said for human  cartilage  production.  first the patient's cartilage   cells are sampled, then  proliferated  and  cultured  on a collagen  protein  scaffold, and finally a full candidates  challengi ng  Trump h uman cartilage is made during 30 days.

added  the  researcher  who  has  worked  at  Royan  Institute,  an  Ira nian  clinical,  research   and ed ucational i nstitute dedicated to biomedical, translational and clinical researches, stern cell research  and infertility treatment.

·The tissue is valid and can be transplanted  to the body if only it can pass the quality control  stages and get a product identification'.'

Hadi, who is currently working as the biological medicines director at Totigh Daru Researi:h and Engineering Company in Tehran, said the transplant is of the 'autologous' type, which is very importa nt in terms of imm une responses, beca use the patient's body does not reject it.

In 2018, a product was produced by human cartilage i n the US and entered the global market. However, being manufactured for each patient separately, it is very expensive.  the resi!archer added.

Hadi further said his MS thesis. titled 'The Effect of Pore Structure of Collagen Scaffold on Cultured Huma n Chondrocytes in Cartilage nssue Engineering' paved the way for developing this research project.

The thesis was then sponsored by Tamin Pharmaceutical I nvestment Company of lran's Social Security Organization and is being continued as a research project, he  added.

•Recently I sent a.n article based on my thesis to the second World Congress on Advanced Biomaterials and Tissue Engineering, and I was i nvited to Rome as the keynote speaker and the chairman of the congress's Scientific Committee.

"My project attracted some reputable pharmaceutical  companies participating  in the congress  due to implementing  innovative methods  in research  and having  a b€tter  quality com pared  to  its US counterpart.